materiały: Skeletal muscle

Evidence: 59 • authority: 92

Spatiotemporal analysis of dystrophin expression during muscle repair.. Background Dystrophin mRNA is produced from a very large genetic locus and transcription of a single mRNA requires approximately 16 h. This prolonged interval between initiation and completion results in unusual transcriptional behaviour: in skeletal muscle, myonuclei express dystrophin continuously and robustly, yet degrade mature transcripts shortly after completion. Consequently, most dystrophin mRNA is nascent, not mature. This implies expression is principally controlled post-transcriptionally, a mechanism that circumvents transcriptional delay, allowing rapid responses to change in demand. Dystrophin protein is ho…

Evidence: 53 • authority: 72

Skeletal muscle (commonly referred to as muscle) is one of the three types of vertebrate muscle tissue, the others being cardiac muscle and smooth muscle. They are part of the musculoskeletal system, and typically are attached by tendons to the bones of a skeleton. The skeletal muscle cells (myocytes) are much longer than in the other types of muscle tissue, and are also known as muscle fibers. The tissue of a skeletal muscle is striated – having a striped appearance due to the arrangement of the sarcomeres. A skeletal muscle contains multiple fascicles – bundles of muscle fibers. Each individual fiber and each muscle is surrounded by a type of connective tissue layer of fascia. Muscle fibe…

Evidence: 47 • authority: 80

The complex pericentriolar material 1 protein allows differentiation between myonuclei and nuclei of satellite cells of the skeletal muscle

Evidence: 47 • authority: 92

The repair capacity spectrum of human skeletal muscle injury from sports to surgical trauma settings.. Skeletal muscle injury and repair have been a major research focus for more than a century. Muscle injuries are defined by their cause and anatomical location and lie on a spectrum in terms of repair outcomes. From contraction-induced necrosis, which initiates regenerative myogenesis for complete restoration of tissue architecture and function to, at the other end of the spectrum, traumatic volumetric muscle loss (VML), where substantial portions (or the whole) of a muscle are lost, leaving the patient with permanent physical disability. Strain injuries are found between these two extremes…

Evidence: 47 • authority: 92

Aging induces sarcopenia by disrupting the crosstalk between the skeletal muscle microenvironment and myofibers.. Background Sarcopenia is a degenerative muscular disease associated with aging, characterized by a reduction in muscle mass and strength. This disease poses a significant global health challenge, owing to its high prevalence and association with adverse outcomes such as increased frailty, impaired physical function, and elevated mortality risk. A deeper understanding of its underlying mechanisms is urgently warranted for the development of effective therapeutic interventions. Aim of review Skeletal muscle is a heterogeneous tissue composed of various cellular components, includi…

Evidence: 42 • authority: 88

Positioning nuclei at the periphery of skeletal muscle cells. Positionnement des noyaux à la périphérie des cellules musculaires Les mouvements nucléaires sont importants pour une multitude de fonctions cellulaires et sont induits par des forces produites par des protéines moteurs et le cytosquelette. Lors de la formation et régénération de myofibres, les noyaux migrent du centre à la périphérie de la cellule pour son bon fonctionnement. De plus, certaines maladies musculaires sont caractérisées par une accumulation de noyaux centraux. En utilisant une approche théorique et empirique, nous démontrons que le mouvement de noyaux vers la périphérie des myofibres est induit par des forces centr…

Evidence: 42 • authority: 92

Endurance training promotes chromatin closure and timely repression of the post-exercise immediate early stress response.. Objectives Endurance training is known to elicit numerous changes in skeletal muscle to enhance performance and function. Many of these adaptations are controlled by the modulation of transcriptional programs in myonuclei. While previous studies have explored alterations in DNA methylation and histone modifications in response to exercise, the specific changes in chromatin restructuring and accessibility, a prerequisite for transcription, are still poorly understood. Methods A multi-omics analysis was performed: ATAC-sequencing was used to map chromatin accessibility in…

Evidence: 36 • authority: 88

Dynamics, specialization and function of muscle nuclei in skeletal muscle. Dynamique, spécialisation et fonction des noyaux musculaires dans le muscle squelettique Le muscle squelettique est un syncytium multinucléé soumis à des contraintes mécaniques et métaboliques continues. Les myonucléus n’y constituent pas une population stable : ils sont soumis à un renouvellement continu en conditions homéostatiques. Cette dynamique n’est pas homogène le long de la fibre, avec une accélération marquée au niveau de la jonction myotendineuse. Le stress oxydant contribue au déclenchement de l’élimination nucléaire, tandis que l’autophagie en assure le mécanisme effecteur. L’altération de ce processus f…

Evidence: 36 • authority: 88

Nuclei isolation from human skeletal muscle v1. This protocol describes a methond of single nucleus isolaton from human skeletal muscle for snRNA sequencing.

Evidence: 36 • authority: 88

Nuclei isolation from human skeletal muscle v1. A protocol for isolating single nuclei from human skeletal muscle. The isolated nuclei can be used for RNAseq or ATACseq.

Evidence: 36 • authority: 92

Cell Death in Skeletal Muscle Diseases: Diverse Roles and Pathological Processes.. Skeletal muscle is vital for movement and metabolism, and its dysfunction underpins disorders like muscular dystrophy and sarcopenia, severely impacting life quality. In these diseases, various cell death pathways are pivotal, driving core pathological features such as fiber loss and chronic inflammation. This study reviews the central role of cell death in skeletal muscle diseases, and analyzes its roles and mechanisms in genetic muscle disorders such as Duchenne muscular dystrophy (DMD), glycogen storage diseases (GSD), mitochondrial myopathies, as well as acquired muscle disorders such as idiopathic inflam…