International Journal of Molecular Medicine

Evidence: 64 • authority: 69

Growth factors and/or angiogenic factors are supposed to improve wound healing. The aim of our study was to evaluate the effects of subcutaneous pretreatment with combinatory proangiogenic factors on wound closure, mechanical properties, vessel density and morphology. Twenty-eight Balb/c mice were divided equally into two groups. A mixture of VEGF (35.0 µg), bFGF (2.5 µg) and PDGF (3.5 µg) was administered subcutaneously 3, 5 and 7 days to 14 mice before full thickness skin punch biopsy wounding, whereas 14 control animals received three injections of 0.2 ml saline solution. Wound sizes were assessed daily and the repaired tissues were harvested 7 days after complete wound closure. Complete closure (≥95% healing of initial wound area) was reached in all proangiogenic pretreated animals on day 10, whereas controls needed 13 days for complete closure. Tensile strengths were nearly twofold higher compared to the controls (p≤0.01). The punch biopsy material revealed 4.2-fold higher vessel densities in the proangiogenic pretreated group. On day 17, the vessel densities in the proangiogenic pretreated wounds were also 3.2‑fold higher compared to the untreated controls. No significant differences were seen in the collagen ratio. Pretreatment with proangiogenic factors revealed several significant effects on wound healing: faster time to closure, a higher vessel density and a better functional outcome. These results suggest a beneficial effect of pretreatment with combinatory growth factors in mouse skin wounds without impaired wound healing. This might be exploited in further investigations in diabetic healing as a therapeutic approach for elective surgery. International Journal of Molecular Medicine Priming with a combination of proangiogenic growth factors improves wound healing in normoglycemic mice

Evidence: 42 • authority: 90

Human Umbilical Cord Perivascular Cells (HUCPVCs): a mesenchymal cell source for dermal wound healing. Mesenchymal stern cells, derived from bone marrow (BM-MSC), have been used with clinical success to enhance the healing of acute and chronic dermal wounds. However, BM-MSC procurement is limited due to the necessity for volunteers of appropriate age. Human Umbilical Cord Perivascular Cells (HUCPVCs) represent an alternative, non-controversial source of postnatal progenitor cells. The goal of the present work was to evaluate the effectiveness of PKH-67 labeled 1PUCPVCs to repair full thickness excisional skin wounds in an immunocompromised mouse model by their direct delivery using a fibrin…

Evidence: 41 • authority: 88

THE STUDY ON EFFICACY OF BUBALINE FIBRIN GLUE ON FULL-THICKNESS PINCH AND PUNCH SKIN GRAFTING IN PORCINE. Skin transplantation or grafting is the principle choice for treating the severe skin losing injury over hundred years. Pinch and punch grafting which is indicated for the treatment of granulating wound, low-grade infection wound and irregular surface wound. Fibrin glue which is the combination of fibrinogen and thrombin has been developed to use as an adhesive for skin graft surgery. In this research used bubaline fibrinogen had the highest fibrinogen concentration and qualification which was extracted by cryoprecipitation and cryocentrifugation. For bubaline fibrin glue application, a…

Evidence: 38 • authority: 92

Carbonic Anhydrase VI in Skin Wound Healing Study on <i>Car6</i> Knockout Mice.. Carbonic anhydrases (CAs) contribute to tumor cell migration by generating an acidic environment through the conversion of carbon dioxide to bicarbonate and a proton. CA VI is secreted to milk and saliva, and it could contribute to wound closure, as a potential trophic factor, in animals that typically lick their wounds. Our aim was to investigate whether human CA VI improves skin-wound healing in full-thickness skin-wound models. The effect was studied in Car6 - / - knockout mice and wild type littermates. Half of both mice strains were given topically administered, milk-derived CA VI after wounding and eight…

Evidence: 35 • authority: 72

A rabies vaccine is a vaccine used to prevent rabies. There are several rabies vaccines available that are both safe and effective. Vaccinations must be administered prior to rabies virus exposure or within the latent period after exposure to prevent the disease. Transmission of rabies virus to humans typically occurs through a bite or scratch from an infectious animal, but exposure can occur through indirect contact with the saliva from an infectious individual. Doses are usually given by injection into the skin or muscle. After exposure, the vaccination is typically used along with rabies immunoglobulin. It is recommended that those who are at high risk of exposure be vaccinated before po…

Evidence: 31 • authority: 80

C57BL/6 mice are resistant to tumor promotion by full thickness skin wounding

Evidence: 29 • authority: 90

Reconstructed Human Skin Models to Study Superficial and Deep Skin Wound Healing In Vitro. Wound healing is an essential and complex biological mechanism to repair barrier breaches in the human body, but it results in scar formation. The extent of scar formation is associated with the depth of injury. Stromal cells play a vital role in wound healing and scar formation, but the role of the subcutaneous tissue in human skin wound healing remains largely unknown. In order to dissect the role of dermal fibroblasts, adipose stromal cells, and adipocytes in superficial and deep skin wound healing, we created a human tissue-engineered skin model and assessed healing outcomes in vitro. Three differ…

Evidence: 0 • authority: 69

Evidence: 0 • authority: 69

Evidence: 0 • authority: 69

Evidence: — • authority: 50